Education

Research Description

Dr. de los Reyes' research focuses on biological processes and combines modeling, bioreactor experiments, and molecular microbial ecology tools in addressing fundamental and practical issues in environmental biotechnology and environmental engineering. Another research focus is sanitation in developing countries. He is interested in two main areas: the interface between microbial ecology and environmental engineering, and global sanitation. At the ecology-engineering interface, molecular techniques are utilized as powerful tools for the analysis of microbial populations in engineered and natural treatment systems. Environmental process engineering is approached from a fundamental standpoint, combining the insights from molecular (DNA- and RNA-based) approaches with innovative process experiments and modeling. In global water, sanitation, and hygiene (WaSH), improving the technologies along the entire sanitation chain is the main focus. His team has developed a new technology for safely and hygienically emptying pit latrines in Africa and Asia. His recent research includes structure-function studies in wastewater treatment systems, landfills, soils, and decentralized sanitation systems.

Honors and Awards

• Alumni Association Distinguished Undergraduate Professor, 2022
• BCEEM, AAEES, 2021
• WEF Fellow, 2021
• Patents for Humanity Award, US Patents Office, 2020
• Corresponding Member, National Academy of Science and Technology, Philippines, 2020
• NC State Academy of Excellence in Global Engagement, 2019
• RELX Group Environmental Challenge Award (for the Flexcrevator), 2018
• NC State Outstanding Teacher Award, 2018
• AAEES Excellence in Environmental Engineering and Science Research, Honor Award, 2017
• Steven K. Dentel/AEESP Global Outreach Award, 2017
• WEF Gordon Fair Outstanding Engineering Educator Award, 2015
• Univ. of the Philippines Los Banos Outstanding Alumnus, 2015
• UP Alumni Association of America Outstanding Alumnus, 2015
• NC State Alumni Outstanding Extension Award, 2015
• NC State Academy of Outstanding Faculty in Extension and Engagement, 2015
• Marist School Outstanding Alumnus Award, 2015
• University Faculty Scholar, 2014
• Outstanding Global Engagement Award, 2014
• AEESP (Assoc. of Environmental Engineering and Science Professors) Service Award, 2013
• Transatlantic Partnership in Excellence in Engineering Scholarship, 2013
• NCSU At Home in the World Faculty Fellow, 2013
• Koh Lectureship Award in Engineering, Philippine-American Academy of Scientists and Engineers, 2013
• Jackson Rigney International Service Award, 2012
• Outstanding Alumnus of Philippine Science HS for Environmental Engineering and Biotechnology, 2011
• Iowa State University Outstanding Young Alumnus Award, 2010
• NCSU Earthwise Award, 2010
• TED Fellow, 2009
• NSF CAREER Award, 2000

Publications

Effect of removal of inhibitors on microbial communities and biogas yield of Jatropha curcas seeds during continuous anaerobic digestion

Haq, A., Malik, A., Khan, A., Weaver, J. E., Wang, L., Khan, H., … Badshah, M. (2023), JOURNAL OF CLEANER PRODUCTION, 426. https://doi.org/10.1016/j.jclepro.2023.139154

Growth of Dunaliella viridis in multiple cycles of reclaimed media after repeated high pH-induced flocculation and harvesting

Lai, Y.-C., Ducoste, J. J., & de los Reyes III, F. L. (2023), SCIENCE OF THE TOTAL ENVIRONMENT, 891. https://doi.org/10.1016/j.scitotenv.2023.164087

Timing and Trends for Municipal Wastewater, Lab-Confirmed Case , and Syndromic Case Surveillance of COVID-19 in Raleigh, North Carolina

Kotlarz, N., Holcomb, D. A., Pasha, T., Reckling, S., Kays, J., Lai, Y.-C., … Harris, A. (2023), AMERICAN JOURNAL OF PUBLIC HEALTH, 113(1), 79–88. https://doi.org/10.2105/AJPH.2022.307108

Using Detrending to Assess SARS-CoV-2 Wastewater Loads as a Leading Indicator of Fluctuations in COVID-19 Cases at Fine Temporal Scales: Correlations Across Twenty Sewersheds in North Carolina

Hoffman, K., Holcomb, D., Reckling, S., Clerkin, T., Blackwood, D., Beattie, R., … Christensen, A. (2023, June 5). , . https://doi.org/10.31223/X5967X

Comparing Rates of Change in SARS-CoV-2 Wastewater Load and Clinical Cases in 19 Sewersheds Across Four Major Metropolitan Areas in the United States

Al-Faliti, M., Kotlarz, N., McCall, C., Harris, A. R., Smith, A. L., Stadler, L. B., … Vela, J. D. (2022), ACS ES&Amp;T Water, 7. https://doi.org/10.1021/acsestwater.2c00106

Diving Deeper Into Food Waste

Reyes, III. (2022, August 11). , . https://doi.org/10.52750/718447

Enhancement of biogas yield during anaerobic digestion of Jatropha curcas seed by pretreatment and co-digestion with mango peels

Haq, A., Khan, A., Haji, K., Khan, S., Shah, A. A., Hasan, F., … Badshah, M. (2022), BIOMASS CONVERSION AND BIOREFINERY, 12(5), 1595–1603. https://doi.org/10.1007/s13399-020-01064-7

Influence of Inoculum Type on Volatile Fatty Acid and Methane Production in Short-Term Anaerobic Food Waste Digestion Tests

Ding, H., Barlaz, M. A., de los Reyes III, F. L., & Call, D. F. (2022, December 9), ACS SUSTAINABLE CHEMISTRY & ENGINEERING, Vol. 12. https://doi.org/10.1021/acssuschemeng.2c04080

Meeting the Water and Sanitation Challenges of Underbounded Communities in the US

Wells, E. C., Vidmar, A. M., Webb, W. A., Ferguson, A. C., Verbyla, M. E., Reyes, F. L., … Mihelcic, J. R. (2022), ENVIRONMENTAL SCIENCE & TECHNOLOGY, 56(16), 11180–11188. https://doi.org/10.1021/acs.est.2c03076

Multifaceted Assessment of Wastewater-Based Epidemiology for SARS-CoV-2 in Selected Urban Communities in Davao City, Philippines: A Pilot Study

Otero, M. C. B., Murao, L. A. E., Limen, M. A. G., Caalim, D. R. A., Gaite, P. L. A., Bacus, M. G., … Del Mundo, D. M. N. (2022), INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 19(14). https://doi.org/10.3390/ijerph19148789

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Grants

NSF IRES Track I: Filling the Gaps in WaSH Research through Field-based Student Research Experiences in Malawi

National Science Foundation (NSF)(4/01/23 - 3/31/26)

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NSF IRES Track I: Filling the Gaps in WaSH Research through Field-based Student Research Experiences in Malawi

Sponsor: National Science Foundation (NSF)

Start Date: 4/01/23

End Date: 3/31/26

Abstract

This project is to provide an 8-week international research experience for NC State students to work in Malawi, with partners from Malawi University of Science and Technology and Mzuzu University. The research will focus on research gaps in Water, Sanitation, and Hygiene (WaSH).

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Support for CDC National Wastewater Surveillance System (NWSS)

Centers for Disease Control & Prevention(1/01/21 - 6/30/21)

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Support for CDC National Wastewater Surveillance System (NWSS)

Sponsor: Centers for Disease Control & Prevention

Start Date: 1/01/21

End Date: 6/30/21

Abstract

North Carolina has been selected as one of eight pilot states in the Centers for Disease Control and Prevention (CDC) National Wastewater Surveillance System (NWSS). This system will provide information on the presence and persistence of SARS-CoV-2-like viruses in wastewater systems as a metric of community COVID-19 prevalence. This approach provides a relatively low-cost way to measure both symptomatic and asymptomatic COVID-19 infections without dependence on supply chains for clinical testing supplies or access to clinical testing. Wastewater surveillance can demonstrate trends in COVID-19 prevalence, direct action to protect public health, and allay concerns about the burden of disease when SARS-CoV-2 concentrations are low. NC State University will support this project by collecting, preparing, and shipping samples to the UNC laboratory (Rachel Noble, PI).

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Improving Wastewater Surveillance for SARS-CoV-2

City of Houston(3/01/20 - 12/30/20)

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Improving Wastewater Surveillance for SARS-CoV-2

Sponsor: City of Houston

Start Date: 3/01/20

End Date: 12/30/20

Abstract

Results from two recent studies have highlighted the value of analyzing wastewater sludge in addition to influent wastewater for tracking SARS-CoV-2 community spread. In a wastewater treatment plant in New Haven, CT, viral RNA concentrations in primary sludge samples were significantly higher than what has been reported in the literature for influent wastewater, even after accounting for a typical concentration factor in primary clarifiers (Peccia et al. 2020). Thickened activated sludge from a wastewater treatment plant in Ourense, Spain was a more sensitive measure of increases in SARS-CoV-2 concentrations than influent wastewater (Balboa et al. 2020). Therefore, the City of Houston may benefit from collecting and analyzing waste activated sludge samples for SARS-CoV-2 in addition to their ongoing monitoring of the virus in influent wastewater.

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RAPID: Monitoring for SARS-CoV-2 in municipal wastewater and sewage to elucidate infection dynamics across major metropolitan areas of the United States

National Science Foundation (NSF)(5/15/20 - 4/30/21)

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RAPID: Monitoring for SARS-CoV-2 in municipal wastewater and sewage to elucidate infection dynamics across major metropolitan areas of the United States

Sponsor: National Science Foundation (NSF)

Start Date: 5/15/20

End Date: 4/30/21

Abstract

The primary goal of the proposed research is to elucidate COVID-19 infection dynamics by monitoring for SARS-CoV-2 RNA in wastewater and sewage in four major metropolitan areas of the US. Influent wastewater (~500 mL) and primary solids (~40 mL) will be collected at wastewater treatment plants serving populations > 100,000 in Los Angeles, Raleigh, Houston and Washington DC. Whenever possible, we will also collect sewage samples within the sewerage network to provide greater resolution of infection dynamics across a city. Samples will be stored at -80 C and analyzed by RT-qPCR when labs are open again. Normalization of SARS-CoV-2 RNA concentrations in wastewater to per capita mass loads using daily flow and population served will provide community-scale information on COVID-19 infection.This coordinated effort across four cities is a unique strength of this work. We hypothesize that the value of wastewater monitoring to track infection dynamics will be influenced by local factors (e.g., whether there is a combined or separate sewerage system, weather events, and wastewater strength) and therefore we chose four cities that represent a wide range of those parameters. There is also a wide range in diagnostic testing capacity across the four cities, with DC having done the most tests per capita and Houston the fewest. The value added of sewage surveillance to clinical diagnostic testing is likely greater in cities with lower testing capacity; we will use our data to test this hypothesis.

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Intensification of Anaerobic Digestion: Co-Digestion of Grease Interceptor Waste (GIW) with Thermally Hydrolyzed (TH) Biosolids

NCSU-WRRI Urban Water Consortium(7/01/20 - 5/01/22)

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Intensification of Anaerobic Digestion: Co-Digestion of Grease Interceptor Waste (GIW) with Thermally Hydrolyzed (TH) Biosolids

Sponsor: NCSU-WRRI Urban Water Consortium

Start Date: 7/01/20

End Date: 5/01/22

Abstract

While significant research has been conducted to date on the benefits and process limitations of co-digestion of GIW with municipal biosolids in a traditional anaerobic digestion process, there is very little knowledge on the benefits and limitations of co-digestion of GIW with municipal biosolids in a process that includes thermal hydrolysis pretreatment process (THP) upstream of the anaerobic digestion process. The proposed study would focus on understanding the benefits and process limitations of co-digestion of GIW with municipal biosolids that have been pretreated with THP. This study will also consider how GIW addition shifts the nutrient concentrations in the anaerobic digestion process and how the reduction in nutrient concentrations impacts microbial population and kinetics in the anaerobic digestion process (i.e., does reduced nutrient content result in less stress on the microbial population in the anaerobic digestion process). This research will benefit the City of Raleigh and other utilities that are considering implementing THP to enhance their digestion process to understand the benefits and limitations of GIW co-digestion with this process. This research will also help to provide much needed information for full-scale implementation of GIW co-digestion in a THP + MAD process, which would ultimately result in an additional outlet option for GIW.

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Tertiary Anammox Filters for Sustainable Nitrogen Removal

NCSU Water Resources Research Institute(3/01/20 - 12/31/21)

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Tertiary Anammox Filters for Sustainable Nitrogen Removal

Sponsor: NCSU Water Resources Research Institute

Start Date: 3/01/20

End Date: 12/31/21

Abstract

Wastewater treatment with Anaerobic Ammonium Oxidation (Anammox) holds the promise of significantly reducing energy and chemical costs associated with nitrogen removal from wastewater The Anammox process involves the anaerobic conversion of nitrite to nitrogen gas with ammonium as the electron donor. While used more regularly in side-stream applications, the use of mainstream Anammox is still limited. Most of the challenges associated with widespread application of Anammox for mainstream nitrogen removal involve the Anammox bacteria being outcompeted by other, more robust organisms commonly found in wastewater and utilized for conventional nitrogen removal. However, in addition to process challenges there are also significant costs associated with a change in infrastructure. One novel possibility that may address both challenges would be to convert existing filter infrastructure into tertiary Anammox filters for mainstream nitrogen removal. For the past two years our research team operated a preliminary pilot Anammox filter at the Neuse River Resource Recovery Facility in Raleigh, NC. While the filter showed exciting promise (> 90% TIN removal in some cases), the controlled nature of the influent into our preliminary pilot filter limited the applicability of the results. This study would build on our experience, and push the frontier of research on sustainable nitrogen removal in wastewater treatment. The primary objective of this study is to compare a pilot scale tertiary Anammox filter to a traditional denitrification filter, under a range of realistic operating conditions. We hypothesize that the Anammox filter will lead to significant cost savings while achieving similar or superior results to a traditional denitrification filter.

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Commercial Partner Engagement for Trash Excluder

Bill and Melinda Gates Foundation(7/25/19 - 2/28/23)

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Commercial Partner Engagement for Trash Excluder

Sponsor: Bill and Melinda Gates Foundation

Start Date: 7/25/19

End Date: 2/28/23

Abstract

The goal of the project is to develop a low-cost, portable auger-based technology that can reliably and hygienically empty a wide variety of pit latrines and septic tanks containing wastes with a range of moisture and trash contents. After extensive field and lab testing, we have finalized an EV design that was successful in emptying pit latrines in the field while successfully excluding trash. Two technologies have been developed: a mechanized trash excluder that actively rejects trash in a pit while allowing emptying of the fecal sludge, and a complete, low-cost system that uses a vacuum and an excluder to empty the de-trashed fecal sludge to pit-side containers (currently called “Flexcrevator”). We also recently finished a generalized market/business analysis for both technologies. NC State’s focus during this part of the project will be to coordinate work with potential commercial partners and contractors to design, build, and test Design Validation (DV) trash excluders. The primary outcomes and results will be: • Design, build a minimum of five (5) DV trash excluder units and create the necessary documentation for Design Validation with an outside contract manufacturer (per New Product Development Guideline) • Testing a minimum of two units (2) units in Ghana with an interested commercial partner (iDE Ghana/Sama Sama) over the course of at least 7 months as a means to understand the performance of the unit and collect commercialization data. This information will be used to develop business model documentation in Ghana, including supply chain, marketing, financing, sales plan. • Testing of the two (2) units will also include a study led by NC State on gender impacts: how the trash excluder operation, access, ownership/business operation, and the how the business of pit emptying is affected by gender. The goal is to identify barriers to inclusive use of the device and define explicit ways to remove these barriers.

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Planning Grant: Engineering Research Center on Sanitation and Water Infrastructure of the Future for Marginalized Communities (SWIFt-MC)

National Science Foundation (NSF)(9/01/19 - 8/31/21)

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Planning Grant: Engineering Research Center on Sanitation and Water Infrastructure of the Future for Marginalized Communities (SWIFt-MC)

Sponsor: National Science Foundation (NSF)

Start Date: 9/01/19

End Date: 8/31/21

Abstract

This is for an NSF ERc Planning Grant to prepare for an ERC proposal. SWIFT-MC’s vision is a future of sanitation and water infrastructure that supports the well-being of marginalized communities and supports sustainable development. These communities disproportionately bear the burden of environmental and health risks, and are not typically prioritized by advances in engineering and technology. To address this complex societal problem, SWIFT-MC will innovate sanitation and water infrastructure (SWI) through deep collaboration across disciplines (e.g., engineering, social and behavior sciences, public health) and active partnership and engagement with marginalized communities, policy makers, and technology designers and developers. As a result, the SWI will be designed, implemented, and maintained equitably to serve the needs of marginalized communities with unique community characteristics and constraints and to achieve desired system properties such as efficiency, flexibility, environmental sustainability, and resiliency.

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Developing High-Yield, Resilient Anaerobic Co-Digestion of Alternative Wastes

Environmental Research & Education Foundation(10/01/18 - 12/31/21)

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Developing High-Yield, Resilient Anaerobic Co-Digestion of Alternative Wastes

Sponsor: Environmental Research & Education Foundation

Start Date: 10/01/18

End Date: 12/31/21

Abstract

Anaerobic digestion (AD) or co-digestion of food waste provides the dual benefits of enabling the sustainable production of energy while providing an alternative disposal option for these ubiquitous wastes. The key to economically feasible AD of food wastes is maintaining high and stable methane yields under various loading and substrate conditions. However, the interactions between substrate variability (different food wastes, loading) and microbial community adaptations are not known, even though these directly impact process resilience and resistance. This is an important issue in full scale operation, since the collected food waste can vary in type, strength, and characteristics on a seasonal, daily, or per load basis. The overall objective of this project is to understand substrate-community interactions to optimize anaerobic digestion of food waste, particularly to increase process resilience and resistance to varying waste types and loads. This will lead to operational procedures that can be used in full-scale implementation of AD of food wastes by municipal utilities and industry.

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Quantifying Metabolically Active Ammonia-Oxidizing Bacteria and Archaea in Complex Soil Microbial Communities using Activity-Based Fluorescent Labeling

BASF Corporation(1/01/18 - 12/31/19)

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Quantifying Metabolically Active Ammonia-Oxidizing Bacteria and Archaea in Complex Soil Microbial Communities using Activity-Based Fluorescent Labeling

Sponsor: BASF Corporation

Start Date: 1/01/18

End Date: 12/31/19

Abstract

Our overall aim is to develop a simple, activity-based fluorescent labeling approach for selectively quantifying metabolically active ammonia-oxidizing bacteria (AOB) and archaea (AOA) in complex soil microbial communities. These specialized microorganisms obtain all of their energy from the oxidation of ammonia (NH3) and initiate this process through the activity of ammonia monooxygenase (AMO). Collectively, these microorganisms can have large impacts on the fertilizer applications to soils and can substantially decrease fertilizer N availability to crop plants. A simple and reliable method that can predict in situ rates of AMO activity in AOA and AOB could be a useful management tool that could help decrease fertilizer waste and environmental impacts of excess fertilizer applications.

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