Education

Research Description

Dr. Ducoste is interested in the operation, design, and optimization of drinking water and wastewater treatment processes. He achieves these goals by incorporating novel experimental techniques and validated numerical models in the analysis of unit processes. He has research experience in the operation and design of chemical mixing, flocculation, sedimentation, and chemical and UV disinfection processes in drinking water treatment. Dr. Ducoste is an expert in modeling water and wastewater treatment process fluid mechanics using Computational Fluid Dynamics (CFD). He has developed CFD models for analysis of chemical and UV disinfection reactors, rapid mix chambers, flocculation basins, filtration systems, wastewater activated sludge systems, waste stabilization ponds, secondary clarifiers, and food waste systems. Dr Ducoste is also interested in modeling cellular regulatory and metabolic pathways and interfacing product formation to produce bioreactor models using CFD.

Publications

Dynamic Modeling of Microalgae Growth and Lipid Production under Transient Light and Nitrogen Conditions

Wang, D., Lai, Y.-C., Karam, A. L., Reyes, III, & Ducoste, J. J. (2019), Environmental Science & Technology, 9. https://doi.org/10.1021/acs.est.9b02908

Flux modeling for monolignol biosynthesis

Wang, J. P., Matthews, M. L., Naik, P. P., Williams, C. M., Ducoste, J. J., Sederoff, R. R., & Chiang, V. L. (2019), Current Opinion in Biotechnology, 56, 187–192. https://doi.org/10.1016/j.copbio.2018.12.003

Genetic Algorithm-Genetic Programming Approach to Identify Hierarchical Models for Ultraviolet Disinfection Reactors

Monroe, J. G., Ducoste, J., & Berglund, E. Z. (2019), JOURNAL OF ENVIRONMENTAL ENGINEERING, 145(2). https://doi.org/10.1061/(ASCE)EE.1943-7870.0001492

Increased loading leads to convergence of microbial communities and high methane yield in adapted anaerobic co-digesters

Wang, L., Hossen, E. H., Aziz, T. N., Ducoste, J. J., & Reyes, III. (2019), Water Research. https://doi.org/10.1016/j.watres.2019.115155

Measuring the Shape and Size of Activated Sludge Particles Immobilized in Agar with an Open Source Software Pipeline

Weaver, J. E., Williams, J. C., Ducoste, J., & Reyes, F. L. (2019), JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (143). https://doi.org/10.3791/58963

Relating nitrogen concentration and light intensity to the growth and lipid accumulation of Dunaliella viridis in a photobioreactor

Lai, Y.-C., Karam, A. L., Sederoff, H. W., Ducoste, J. J., & Reyes, F. L. (2019), Journal of Applied Phycology. https://doi.org/10.1007/s10811-019-01897-4

Assessing the impact of the 4CL enzyme complex on the robustness of monolignol biosynthesis using metabolic pathway analysis

Naik, P., Wang, J. P., Sederoff, R., Chiang, V., Williams, C., & Ducoste, J. J. (2018), PLOS ONE, 13(3), e0193896. https://doi.org/10.1371/journal.pone.0193896

Controlling aerobic biological floc size using Couette-Taylor Bioreactors

Weaver, J. E., Hong, H., Ducoste, J. J., & Reyes, III. (2018), Water Research, 147, 177–183. https://doi.org/10.1016/j.watres.2018.09.060

Development of Photochemical Microsensors for Evaluating Photosynthetic Light Dose Distributions in Microalgal Photobioreactors

Karam, A. L., Reyes, III, & Ducoste, J. J. (2018), Environmental Science & Technology, 10(21), 12538–12545. https://doi.org/10.1021/acs.est.8b02056

Improving wood properties for wood utilization through multi-omics integration in lignin biosynthesis

Wang, J. P., Matthews, M. L., Williams, C. M., Shi, R., Yang, C. M., Tunlaya-Anukit, S., … Shuford, C. M. (2018), Nature Communications, 9. https://doi.org/10.1038/s41467-018-03863-z

View all publications via NC State Libraries

Grants

Development of Test Methods to Characterize Heat Production from Special Wastes Disposed in Landfills

Environmental Research & Education Foundation(9/01/18 - 8/30/20)

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Development of Test Methods to Characterize Heat Production from Special Wastes Disposed in Landfills

Sponsor: Environmental Research & Education Foundation

Start Date: 9/01/18

End Date: 8/30/20

Abstract

Recently, there have been reports of municipal solid waste (MSW) landfills that have been experiencing temperatures in excess of 80 °C. Elevated temperatures have a number of deleterious effects that are well known to landfill owners. Consequently, elevated temperature landfills often require increased monitoring and management. In recent work supported by the EREF, we developed a model of heat accumulation in a landfill. The objective of the model was to help identify and mathematically describe all sources of heat input, generation and loss in a typical Subtitle D landfill. The model simulations identified several reactions that contribute significant heat to landfills including the hydration and carbonation of calcium-containing wastes (e.g., ash) and aluminum corrosion. Model predictions however, were based on information adopted from the literature for systems other than landfills. In addition to MSW, many landfills receive non-hazardous industrial wastes including ash from both coal and MSW combustion, ash used to solidify liquid wastes, auto shredder residue (ASR) that contains Al and Fe, and perhaps other Al-containing wastes. Methods are needed to measure the heat production potential of such wastes under landfill-relevant conditions and to use the resulting heat production data to evaluate the quantity of a given waste that can be disposed without the accumulation of unacceptable heat. The objectives of the proposed research are to (1) develop laboratory methods to measure heat evolution from special wastes under landfill-relevant conditions and (2) measure rates of heat production to parameterize our heat accumulation model. The model will then be used to estimate acceptable quantities of specific heat-producing wastes for disposal. The proposal emphasizes heat release from ash and metal corrosion. However, methods will be generalized to assess the heat generation of other wastes.

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LSAMP BD: North Carolina State University Preparing Researchers of the Future (PROF) NC-LSAMP

National Science Foundation (NSF)(7/01/18 - 6/30/20)

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LSAMP BD: North Carolina State University Preparing Researchers of the Future (PROF) NC-LSAMP

Sponsor: National Science Foundation (NSF)

Start Date: 7/01/18

End Date: 6/30/20

Abstract

The North Carolina Louis Stokes Alliance for Minority Participation (NC-LSAMP), requests a supplement to implement Cohort VII of the “Bridge to the Doctorate” program with North Carolina State University (NC State) serving as the institutional site. NC State University is a member since the beginning of the North Carolina Louis Stokes Alliance for Minority Participation. As one of the two flagship research universities in the University of North Carolina Education System, NC State’s world leadership in research and education makes it an ideal site for this phase of the NC-LSAMP Bridge to the Doctorate program. NC State university proposes to support a critical mass of 12 Bridge to the Doctorate fellows in each of the two years of this program. We have a firm written commitments from senior NC State University leadership in the College of Engineering and College of Science, to guarantee funding for BD Fellows through completion of their Ph.D. degree. We plan to develop new initiatives that will increase the percentage of BD fellows that complete their doctorate. Our proposed initiatives will help recruit, retain, and prepare researchers of the future in STEM beyond the timeline of this BD program and change the culture at NCSU for underrepresented graduate students in STEM. At NC State University, our goals are 1) to broaden participation of underrepresented students pursuing a graduate degree, 2) to improve graduate student mentoring and develop a Presidential style advisory panel structure for each student, and 3) to provide workshops on research methods and transition from undergraduate to graduate school for graduate students.

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Using Microbial Ecology Theory to Understand Microbial Community Dynamics and Improve Function of Anaerobic Bioreactors

National Science Foundation (NSF)(8/01/18 - 7/31/21)

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Using Microbial Ecology Theory to Understand Microbial Community Dynamics and Improve Function of Anaerobic Bioreactors

Sponsor: National Science Foundation (NSF)

Start Date: 8/01/18

End Date: 7/31/21

Abstract

In the continuing quest to relate microbial communities in bioreactors to function and environmental and operational conditions, engineers and biotechnologists have adopted the latest molecular and ‘omic methods. Despite the large amounts of data generated, gaining mechanistic insights and using the data for predictive and practical purposes is still a huge challenge. This project will use a methodological framework to guide experimental design to improve the operation, start-up, and resilience and resistance of anaerobic bioreactors co-digesting food and FOG wastes. This research represents leading edge work to combine molecular microbial methods, bioreactor experiments, and modeling to identify and exploit the underlying factors that govern microbial community assembly in anaerobic co-digestion systems.

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Collaborative Research: Modeling the regulatory network of InsP6 signaling in plants.

National Science Foundation (NSF)(8/15/16 - 12/31/19)

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Collaborative Research: Modeling the regulatory network of InsP6 signaling in plants.

Sponsor: National Science Foundation (NSF)

Start Date: 8/15/16

End Date: 12/31/19

Abstract

Myo-inositol phosphates (InsPs) are signaling molecules that are critically important in a number of developmental, metabolic and signaling processes in eukaryotes. The fully phosphorylated form, inositol hexakisphosphate or InsP6, plays important roles in many eukaryotes. A new frontier for InsP signaling is the study of unique signaling roles for a novel group of InsPs containing diphospho- or triphospho- moieties (PPx) at one or more positions on the Ins ring. In some ways, these PPx-InsPs are analogous to ATP in that they contain high-energy pyrophosphate bonds, and in addition, have been linked to communicating the energy status of the cell in other organisms. In this collaborative project, we previously developed analytical methods to detect and quantify PPx-InsPs in plant tissues, identified and cloned genes encoding the VIP kinases that are responsible for inositol pyrophosphate production in plants, and developed genetic resources to examine function of the Vip genes. Our preliminary data using mutants lacking both Vip genes reveal these genes are key in signaling the energy status of the plant cell. Further, we have identified a possible mechanistic link between inositol pyrophosphate signaling and a major regulator of eukaryotic metabolism, the Sucrose non-fermenting related kinase 1 (SnRK1). Given the immediate need to understand and manipulate plant bioenergy, the long-term goal of this project is to understand how InsP6, InsP7 and InsP8 convey signaling information within the cell. We focus on these molecules in plants, but point out that our model and findings are applicable to understanding the InsP6 signaling hub in other eukaryotes. During the proposed project, we plan to address several unresolved questions pertaining to PPx-InsPs and energy by first adding to a preliminary kinetic model of this signaling pathway.

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Evaluation of Alternative Binder Material to Reduce Sewer Collection system Infrastructure Maintenance and Enhance Sustainability

NCSU Water Resources Research Institute(3/01/16 - 6/30/19)

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Evaluation of Alternative Binder Material to Reduce Sewer Collection system Infrastructure Maintenance and Enhance Sustainability

Sponsor: NCSU Water Resources Research Institute

Start Date: 3/01/16

End Date: 6/30/19

Abstract

In North Carolina, it is estimated that over 10,000 SSOs occur annually, costing hundreds of thousands of dollars in clean-up. Of those blockages, 50% are related to FOG release into the collection system. The amount of FOG that are routinely discharged into the nation’s sanitary sewer systems is increasing as commercial food preparation and serving facilities and high density dwellings increase particularly in metropolitan communities. The migration to urban centers has increased the maintenance required by pretreatment coordinators in efforts to keep the sewer pipes free of FOG deposit related blockages. The statistics of SSOs from FOG deposits and their potential environmental impact indicate the need to examine methods to reduce the accumulation of FOG deposits in the sanitary sewer collection system and enhanced strategies that remove FOG deposit chemical precursors. In addition to sewer collection system problems, North Carolina is dealing with the unfortunate discharge of nearly 40,000 tons of coal ash from a major power provider into a major waterway. Over 5.5 million tons of coal ash is produced in NC, ranking NC 9th in the US in coal ash generation. There are 37 ash ponds in NC and the majority of them are not equipped with leachate collection system to protect groundwater. The increased use of fly ash in infrastructure materials, such as production of pipelines, could introduce a way to offset the fly ash created by coal-fired power plants and contribute to reducing the risk of future pond dam failures and contaminations of water resources. The objectives of the proposed research are to: (1) Evaluate alternative binder materials used in precast concrete that significantly reduces or eliminates calcium leaching, (2) Evaluate the adhesion properties of FOG deposits on these alternative concrete surfaces, and (3) Assess the structural durability of these alternative concrete materials. The proposed research project will be the first to explore the FOG deposit formation potential and adhesive properties of alternative concrete materials using geopolymers. The research results of this project will offer North Carolinian wastewater municipalities with strategies to maintain a sustainable sewer collection system in high density metropolitan cities that are experiencing significant growth and alleviate the potential environmental and public health harm from FOG related SSOs as well as an avenue for offsetting the disposal of fly ash.

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Quantifying the Grease Interceptor Effluent Fatty Acid concentration and Profile: Impact of Pump out Frequency

Emerson Electric Company(8/01/14 - 5/15/16)

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Quantifying the Grease Interceptor Effluent Fatty Acid concentration and Profile: Impact of Pump out Frequency

Sponsor: Emerson Electric Company

Start Date: 8/01/14

End Date: 5/15/16

Abstract

Fat, oil, and grease (FOG) deposits are calcium based saponified solids that clog sewer lines that potentially lead to sanitary sewer overflows (SSOs). SSOs can pose a significant threat to public health due to the exposure of pathogens and other harmful contaminants. While recent research studies have provided new insights into the chemical characteristics of these insoluble FOG deposits as well as the chemical, physical, and environmental factors that influence the amount and rate of their formation, detailed research has yet to be performed that provides a fundamental understanding of how the maintenance schedule and the parameters used to assess the current state of GI readiness for continued FOG separation provides true protection against the formation of FOG deposits in sewer collection systems. Therefore, the objectives of this research study are as follows: 1) Develop a method to determine the concentration and profile of long chain free fatty acids (LCFA) in the GI effluent, 2) Determine the impact of GI pump out frequency on the effluent LCFA profile and concentration, and 3) Determine the effectiveness of the 25% combined solids and FOG layer thickness protocol on the GI effluent LCFA profile and concentration

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Inducing Aerobic Granulation in Continuous-Flow Reactors using Shear Variability

National Science Foundation (NSF)(8/15/13 - 5/31/18)

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Inducing Aerobic Granulation in Continuous-Flow Reactors using Shear Variability

Sponsor: National Science Foundation (NSF)

Start Date: 8/15/13

End Date: 5/31/18

Abstract

We propose to prove that aerobic granulation in lab-, pilot-, and full-scale activated sludge systems can be induced by engineering the bioreactor to have variable shear distribution. This project will thus impact wastewater treatment plant design and operation by increasing settling, improving organic contaminant removal efficiencies, decreasing reactor volume, and increasing organic and nutrient loading.

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EFRI-PSBR:Closing the Loop- Towards a PSBR Design Framework for Self-Sustained Marine Microalgal-Based Fuel Production

National Science Foundation (NSF)(9/01/13 - 8/31/19)

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EFRI-PSBR:Closing the Loop- Towards a PSBR Design Framework for Self-Sustained Marine Microalgal-Based Fuel Production

Sponsor: National Science Foundation (NSF)

Start Date: 9/01/13

End Date: 8/31/19

Abstract

NC State's EFRI PSBR program will model, develop, implement, and evaluate a scalable photosynthetic biorefinery (PSBR) that uses transformational nutrient recycle processes and supports efficient conversion of CO2 to lipid (oil) in a marine microalgae-based system. Algal oils are an ideal feedstock for biofuels production, offering high production density and the ability to use marginal water (municipal wastewater, brackish water, etc.) and reuse CO2 in flue gases. However, there are a number of technical challenges associated with culturing algae in current generation PSBRs. Using a tightly coupled synergistic approach employing both Engineers and Biologists, the team will: a) genetically engineer a marine microalgae species (Dunaliella spp.) with enhanced CO2 uptake/fixation and the capability to recycle N and P from microalgal biomass; b) design a small-scale PSBR informed by our kinetic model which will be used to develop a scalable dynamic reactor model based on computational fluids dynamic simulation of the PSBR; c) develop innovative, scalable approaches for algal harvesting and lipid extraction; and d) develop an analytical framework for the LCA of our microalgal PSBR system to include creation of flexible and scalable cost and LCI process models that will ultimately lead to generation of a robust PSBR life-cycle decision tool that can be applied to this and other PSBR systems. Intellectual Merit New technologies developed as a result of this project for scalable, sustainable culturing of phototrophic marine microalgae for optimized algal oil production will broaden scientific discovery and create the framework, synergy and momentum for biologists and engineers to further explore rational design and operation of PSBRs. Genetic enhancement, reactor modeling, and LCA will be used to optimize production of algal biomass and lipids in our PSBR. Exploration of innovative and efficient means for algal CO2 uptake/fixation, cell harvesting, lipid extraction, and nutrient and water recycle, will transform the scientific development of algae-based biorefineries. Demonstration of novel Lagrangian microsensors that can assess accumulation of light radiation in proportion to its exposure during transport through the reactor will significantly aid in the modeling and testing of PSBR operation in response to light. PSBR design optimization enabled by our experiment-informed kinetic and CFD modeling and LCA will advance knowledge in rational microalgal-based PSBR design and operation, ultimately leading to development of fully scalable and sustainable biofuel feedstock production systems. Broader Impacts The development of truly scalable and sustainable PSBRs offers tremendous economic and environmental impact by reducing the transportation sector?s reliance on fossil fuels. This increases the prospect of finally being able to fully exploit the promise of algae as a biofuels feedstock, given that production of algal-oil derived biofuels that are fully compatible with all existing infrastructure has been demonstrated. Innovative and transformative enabling-technologies that will permit robust production of marine microalgae biomass and lipids in scalable and sustainable PSBRs will bring significant environmental and economic benefits to the nation through the development of an efficient, high-yield alternative energy feedstock production platform. This interdisciplinary research among engineers, microbiologists, molecular biologists and plant physiologists provides unique training opportunities for high school, undergraduate, graduate and postdoctoral scholars to bridge traditional disciplines and become the new generation of scientists and engineers to develop renewable energy for future generations.

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CREATIV Dynamic Regulatory Modeling of the Iron Deficiency Response in Arabidopsis thaliana

National Science Foundation (NSF)(8/15/12 - 7/31/19)

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CREATIV Dynamic Regulatory Modeling of the Iron Deficiency Response in Arabidopsis thaliana

Sponsor: National Science Foundation (NSF)

Start Date: 8/15/12

End Date: 7/31/19

Abstract

In this proposal, we present a novel paradigm for identifying putative cis-regulatory promoter targets that control the regulation of stress responses in plants. This paradigm will also be used to identify critical regulatory components that differentiate the regulatory stress response across different cell types. We first develop the computational and analytical infrastructure needed to build a dynamic model of the gene regulatory network from time-course transcription profile data that quantifies the stress response. Novel analytical model refinement techniques are proposed to reduce the space of feasible solutions, generate specifications for model validation experiments, and test functional redundancy in the response. Parallel computing architectures will be used to scale the implementation of these model refinement approaches to the size and complexity associated with gene regulatory networks. The dynamic model of the gene regulatory network will be used to identify relationships between genes, build corresponding functional modules, and identify putative cis-regulatory promoter targets and regulatory components that can be used to alter responses to biotic and abiotic stresses in plants. Previous cell-specific transcription profiling has indicated that cell types have distinct expression profiles and respond differently to stress. We will generate cell-specific time-course transcription profiles using experiment specifications derived from the dynamic gene regulatory network. These data will be used to create a cell-specific dynamic gene regulatory network for identifying regulators that are key in differentiating the stress response between cell types.

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Assessment of Alternative Herbicides for Roots Intrusion Treatment

RootX Corp.(1/01/11 - 12/31/12)

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Assessment of Alternative Herbicides for Roots Intrusion Treatment

Sponsor: RootX Corp.

Start Date: 1/01/11

End Date: 12/31/12

Abstract

The objective of this study is to determine the efficacy of a proposed herbicide and how it compares to the current RootX formula.

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